Journal: Autophagy

Article Title: Zika virus NS2A protein induces the degradation of KPNA2 (karyopherin subunit alpha 2) via chaperone-mediated autophagy

doi: 10.1080/15548627.2020.1823122

Figure Lengend Snippet: List of primers used in this study

Article Snippet: The primary mouse monoclonal antibodies against KPNA1 (Santa Cruz Biotechnology, sc-101,292), KPNA2 (Santa Cruz Biotechnology, sc-55,537), hemagglutinin (HA) tag (ThermoFisher Scientific, 26,183), GFP (Biolegend, 75,818–584), HSPA8/HSC70 (Santa Cruz Biotechnology, sc-7298), ubiquitin (Santa Cruz Biotechnology, sc-8017), GAPDH (Santa Cruz Biotechnology, sc-365,062), and TUBB1/β-tubulin (Sigma, T7816), and rabbit polyclonal antibodies against ZIKV NS4B (GeneTex, GTX133311), ZIKV E (GeneTex, GTX133314), NS2B (GeneTex, GTX133318), NS5 (GeneTex, GTX133329) and LAMP2A (Boster, M01573) were used in this study.

Techniques:

Journal: The Journal of Infectious Diseases

Article Title: Molecular Diagnostic Field Test for Point-of-Care Detection of Ebola Virus Directly From Blood

doi: 10.1093/infdis/jiw330

Figure Lengend Snippet: List of the Pathogens Used for the Evaluation of the Specificity of Ebola Virus (EBOV) Reverse Transcription–Loop-Mediated Isothermal Amplification (RT-LAMP)

Article Snippet: Final reaction products were visualized by electrophoresis on 2% agarose gels stained with ethidium bromide. table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 Sample Isolate Source Part Number Stock Titer Titer/Test (50 µL) 1 a Marburg virus–Angola UTMB Not available 8 × 10 7 PFU/mL 2 × 10 4 PFU/mL 2 a Marburg virus–Musoke UTMB Not available 4.4 × 10 6 PFU/mL 1.1 × 10 3 PFU/mL 3 a Lassa virus UTMB Not available 1.75 × 10 6 PFU/mL 4.25 × 10 2 PFU/mL 4 a Crimean-Congo hemorrhagic fever virus UTMB Not available 1 × 10 7 PFU/mL 2.5 × 10 3 PFU/mL 5 Respiratory syncytial virus A (2006 isolate) Zeptometrix 0810040ACF 1.95 × 10 7 U/mL TCID 50 5.36 × 10 3 units 6 Respiratory syncytial virus B (CH93(18)-18) Zeptometrix 0810040CF 1.51 × 10 6 U/mL TCID 50 4.16 × 10 2 units 7 Influenza A virus (Wisconsin/67/05) Zeptometrix 0810252CF 1.41 × 10 5 U/mL TCID 50 3.88 × 10 1 units 8 Influenza B virus (Massachusetts/2/12) Zeptometrix 0810239CF 1.41 × 10 5 U/mL TCID 50 3.88 × 10 1 units 9 Salmonella enterica subspecies arizonae ATCC d ATCC 13314 1.30 × 10 8 CFU/vial (1 mL) 3.58 × 10 4 CFU 10 Salmonella enterica serotype Typhi Zeptometrix 0801933 1.89 × 10 9 CFU/mL 5.20 × 10 5 CFU 11 Pseudomonas aeruginosa ATCC ATCC 10145 9.15 × 10 7 CFU/mL 2.52 × 10 4 CFU 12 Salmonella sonnei ATCC ATCC 29930 >10 4 CFU/mL 2.75 × 10 1 CFU 13 Plasmodium vivax ATCC ATCC 30152 4.90 × 10 6 U/mL TCID 50 1.35 × 10 3 units 14 Plasmodium falciparum ATCC ATCC 30932 2.84% parasitemia/vial (0.7 mL) 0.02% parasitemia 15 Dengue virus serotype 1 (Hawaii) Zeptometrix 0810088CF 2.45 × 10 5 U/mL TCID 50 6.74 × 10 1 units 16 Dengue virus serotype 2 (New Guinea) Zeptometrix 0810089CF 3.16 × 10 5 U/mL TCID 50 8.69 × 10 1 units 17 Dengue virus serotype 3 (strain H87) Zeptometrix 0810090CF 1.15 × 10 7 U/mL TCID 50 3.16 × 10 3 units 18 Dengue virus serotype 4 (H241) Zeptometrix 0810091CF 3.39 × 10 7 U/mL TCID 50 9.32 × 10 3 units 19 Vibrio cholerae (Z133) Zeptometrix 0801902 1.90 × 10 10 CFU/mL 5.23 × 10 6 CFU Open in a separate window Abbreviations: ATTC, American Type Culture Collection; CFU, colony-forming units; PFU, plaque-forming units; TCID 50 , 50% tissue culture infective dose; UTMB, University of Texas Medical Branch, Galveston. a Specificity experiments using these pathogens were done at the UTMB in a biosafety level 4 facility.

Techniques: Virus, Amplification

Journal: International journal of molecular sciences

Article Title: Influence of Atherosclerosis-Associated Risk Factors on Expression of Endothelin Receptors in Advanced Atherosclerosis.

doi: 10.3390/ijms26052310

Figure Lengend Snippet: Figure 1. Immunohistochemistry of exemplary tissue specimen. (A–C) Representative slides after Elastica van Gieson staining of a healthy human artery (A) consisting of tunica interna (endothelium), tunica media and tunica externa, (B,C) advanced atherosclerotic plaques with calcification indicating advanced atherosclerosis. The depicted carotid specimen (B) had a NASCET score of 90% and led to symptomatic atherosclerosis. The femoral specimen (C) had a NASCET score of 80% and was also symptomatic. (D) Lateral femoral artery branch showing ETAR expression in the smooth muscle cells of the tunica media. (E) Lateral femoral artery branch showing ETBR expression in the endothelial layer of the tunica intima. Confocal images show in blue: DAPI, red: ETAR/ETBR, 20× magnification.

Article Snippet: Sections of 5 μm thickness were prepared using a microtome (RM2235, Leica; Wetzlar, Germany) and stained for ETAR (Abcam ab76259, 1:100; Abcam Limited, Cambridge, UK) or ETBR (Proteintech 20964- 1-AP, 1:200, ChromoTek GmbH, Planegg-Martinsried, Germany) using a Vectastain kit (PK-6101, Vector Labs; Burlingame, CA, USA) with peroxidase substrate (SK-4100, Vector Labs) or the fluorescent-dye-coupled secondary antibody anti-rabbit Alexa 647 (Invitrogen A32733, 1:200; Thermo Fisher Scientific, MA, USA).

Techniques: Immunohistochemistry, Staining, Expressing